القای فنوتیپ عصبی در سلول‌های بنیادی رویانی توسط داروی دپرنیل

Introduction & Objective:: Previous studies have shown that ES cells could be induced to differentiate into neurons and gelia in vitro. Induction protocols are based on culture in the presence of an inducer such as RA. In this study, the effect of deprenyl on the differentiation of embryonic stem cells (ES) cells to neuron-like cells was investigated. Deprenyl is a type B monoamine oxidase inhibitor which has been used for treatment of Parkinson s disease. The drug protects neurons in a variety of modes, by inducing neurotrophin expression, it causes neuritis outgrowth. These raised the possibility of a direct effect of deprenyl on pleuripotent ES to develop into neurons. Materials & Methods: Cells of an embryonic stem cell line (CCE) were cultured as aggregates (embryoid bodies) then, they were exposed to a concentration of 10-8 M deprenyl. CCE, was used to evaluate the induction of neuronal differentiation by deprenyl. Morphological and immunohistochemical techniques were used to evaluate the differentiation of CCE cells In addition to the Cresyl violet used for morphological study, antinestin antibody was applied to characterize the neuroepithelial differentiation. Moreover, anti-synaptophysin and anti-tyrosine hydroxylase antibodies were used to characterize the neuronal phenotype of ESCs. Results: The results indicated that the concentration of 10-8 M deprenyl could induce the differentiation of ESCs into neurons.